Construction of eco-biosensor and its potential application for highly selective, sensitive and fast detection of viscumin

Viscum album lectin 1 (Viscumin) is one of the most important plant-based protein of potential adjuvant in cancer treatment. Therefore, the use of nano-biosensor technology as a novel emerges of biosensors is crucial to detect this modal agent in pharmacological study. Molecular imprinted polymer using 9-mer peptides sequence (epitope) was applied as a template. Using ultraviolet light, hydrogen bonding attained between the functional monomer and epitope, leading to the formation of a molecularly imprinted polymer. In the following, the epitope was derived from the surface of the polymer by sodium dodecyl sulfate (SDS) 2.5% and acetic acid 0.6% w/w. Finally, the designed nano-biosensor was exposed to different concentrations of the epitope. The selectivity of the nano-biosensor was tested in complex matrices such as blood plasma and urine. The scatchard analysis was covered for a consequence of the dissociation constants and the numbers of binding sites. Based on the results, the designed nano-biosensor has a limit of detection of 0.117 ng/μl and limit of quantification of 0.517 ng/μl in PBS buffer, respectively. These amounts stood 0.5 ng/μl and 0.8 ng/μl for urine environment and 1.25 ng/μl and 5 ng/μl for human blood fresh frozen plasma in the presence of ricin as the most homologue of viscumin (ML1) in fixed concentration (12:1), respectively. The time of detection and optimum pH was 8.0 min and 7.4, respectively. Designed and synthesized nano-biosensor is adequately qualified to be used in diverse complex areas, due to good efficiency.