A novel compound has been synthesised and characterised by various spectroscopic techniques. Analysing the crystal structures of the compound shows that multiple molecular interactions exist. The compound exhibits distinct aggregation-induced emission activity in EtOH/H2O accompanying a 2-fold enhancement of fluorescence intensity at ~609nm. In addition, the cytotoxicity assay and confocal microscopy imaging show that the compound is hypotoxic and can be used as a fluorescent labelling dye in the near-infrared region to track mitochondria in living cells.