蛋白质组
普通小球藻
生物炼制
限制
计算生物学
转录组
焊剂(冶金)
生物
化学
蛋白质组学
生物化学
藻类
生物燃料
生物技术
植物
基因
基因表达
有机化学
工程类
机械工程
作者
Eric P. Knoshaug,Alida T. Gerritsen,Calvin A. Henard,Michael T. Guarnieri
出处
期刊:Methods in molecular biology
日期:2020-01-01
卷期号:: 51-59
标识
DOI:10.1007/978-1-0716-0195-2_5
摘要
Microalgae present promising feedstocks to produce renewable fuel and chemical intermediates, in part due to high storage carbon flux capacity to triacylglycerides or storage carbohydrates upon nutrient deprivation. However, the mechanism(s) governing deprivation-mediated carbon partitioning remain to be fully elucidated, limiting targeted strain engineering strategies in algal biocatalysts. Though genomic and transcriptomic analyses offer key insights into these mechanisms, active post-transcriptional regulatory mechanisms, ubiquitous in many microalgae, necessitate proteomic and post-translational (e.g., phospho- and nitroso-proteomic) analyses to more completely evaluate algal responsiveness to nutrient deprivation. Herein, we describe methods for isolating total algal protein and conducting proteomic, phosphoproteomic, and nitrosoproteomic analyses. We focus on methods deployed for the chlorophyte, Chlorella vulgaris, a model oleaginous alga with high flux to renewable fuel and chemical precursors.
科研通智能强力驱动
Strongly Powered by AbleSci AI