嗜酸乳杆菌
透明质酸
细菌
食品科学
琼脂糖凝胶电泳
乳酸
化学
微生物学
乳酸菌
樱乳杆菌
生物化学
生物
生物技术
益生菌
发酵
基因
遗传学
作者
Fatemeh Fotouhi Chahuki,Saeed Aminzadeh,Vahab Jafarian,Fatemeh Tabandeh,Mahvash Khodabandeh
标识
DOI:10.1016/j.ijbiomac.2018.10.112
摘要
Hyaluronic acid (HA) is a natural polymer with various molecular weights that specify multiple biological roles. Traditionally, HA is obtained from animal waste and conventional pathogenic streptococci. However, there are challenges in these processes such as the presence of exotoxins, hyaluronidase, and viral contamination. In order to reduce these problems, this study was conducted to produce HA using recombinant bacterium that is generally recognized as safe (GRAS), and thereafter increase production through experimental design. At first, some lactic acid bacteria were screened and evaluated for HA production. Accordingly, among the selected bacteria, Lactobacillus acidophilus PTCC1643 produced about 0.25 g HA/L in the 48th hour of cultivation, and was thus selected as an alternative host for heterologous HA production. An expression vector containing HA synthase genes was transformed into L. acidophilus by electroporation. Consequently, HA production increased to 0.4 g/L. Eventually, response surface method (RSM) was used, which increased HA production to 1.7 g/L. This is approximately 7-fold higher than that produced at first. The resulting HA was characterized by FTIR spectroscopy and its molecular weight was estimated using agarose gel electrophoresis. In conclusion, L. acidophilus could be a safe, effective, and novel HA producer with industrial potential and commercial prospects.
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