显微镜
显微镜
全内反射荧光显微镜
荧光显微镜
活体细胞成像
超分辨显微术
显微术
光学
薄层荧光显微镜
帧速率
计算机科学
分辨率(逻辑)
材料科学
荧光
生物
物理
扫描共焦电子显微镜
细胞生物学
人工智能
遗传学
细胞
作者
Peter Kner,Bryant B. Chhun,Eric R. Griffis,Lukman Winoto,Mats G. Gustafsson
出处
期刊:Nature Methods
[Springer Nature]
日期:2009-04-26
卷期号:6 (5): 339-342
被引量:707
摘要
The use of a spatial light modulator for illuminating the sample in structured-illumination microscopy (SIM) increases imaging speed by three orders of magnitude. The resulting 100-nm resolution and 11-Hz frame rate allowed video imaging of tubulin polymerization and depolymerization as well as kinesin movement on microtubules. Structured-illumination microscopy can double the resolution of the widefield fluorescence microscope but has previously been too slow for dynamic live imaging. Here we demonstrate a high-speed structured-illumination microscope that is capable of 100-nm resolution at frame rates up to 11 Hz for several hundred time points. We demonstrate the microscope by video imaging of tubulin and kinesin dynamics in living Drosophila melanogaster S2 cells in the total internal reflection mode.
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