Human Papillomavirus (HPV) DNA Copy Number Is Dependent on Grade of Cervical Disease and HPV Type

宫颈上皮内瘤变 宫颈癌 生物 拷贝数变化 乳头瘤病毒科 基因型 病毒学 医学 分子生物学 癌症 基因 遗传学 基因组
作者
David C. Swan,Ruth Ann Tucker,Guillermo Tortolero‐Luna,Michele Follen Mitchell,Louise Wideroff,Elizabeth R. Unger,Rosane Nisenbaum,William C. Reeves,Joseph P. Icenogle
出处
期刊:Journal of Clinical Microbiology [American Society for Microbiology]
卷期号:37 (4): 1030-1034 被引量:243
标识
DOI:10.1128/jcm.37.4.1030-1034.1999
摘要

ABSTRACT The association between human papillomavirus (HPV) DNA copy number and cervical disease was investigated. Viral DNA copy number for the most common high-risk HPV types in cervical cancer (types 16, 18, 31, and 45) was determined in cervical cytobrush specimens from 149 women with high-grade cervical intraepithelial neoplasias (CIN II-CIN III), 176 with low-grade CIN (CIN I), and 270 with normal cytology. Quantitative, PCR-based fluorescent assays for each of the HPV genotypes and for the β-globin gene were used. The amount of cellular DNA increased significantly with increasing disease; thus, HPV was expressed as copies per microgram of cellular DNA. The assay had a dynamic range of >10 7 , allowing documentation for the first time of the wide range of HPV copy numbers seen in clinical specimens. Median HPV DNA copy number varied by more than 10 4 among the viral types. HPV16 was present in the highest copy number; over 55% of HPV16-positive samples contained more than 10 8 copies/μg. Median copy number for HPV16 showed dramatic increases with increasing epithelial abnormality, an effect not seen with the other HPV types. HPV16 increased from a median of 2.2 × 10 7 in patients with normal cytology, to 4.1 × 10 7 in CIN I patients, to 1.3 × 10 9 copies/μg in CIN II-III patients. Even when stratified by cervical disease and viral type, the range of viral DNA copies per microgram of cellular DNA was quite large, precluding setting a clinically significant cutoff value for “high” copy numbers predictive of disease. This study suggests that the clinical usefulness of HPV quantitation requires reassessment and is assay dependent.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
英姑应助怡然的烤鸡采纳,获得10
1秒前
1秒前
着急毕业的干饭人完成签到,获得积分10
1秒前
DD完成签到,获得积分20
1秒前
lc发布了新的文献求助10
1秒前
2秒前
蓝桉发布了新的文献求助10
3秒前
夜盏丿发布了新的文献求助10
3秒前
4秒前
坚定迎天发布了新的文献求助10
5秒前
北风歌完成签到,获得积分10
5秒前
plant发布了新的文献求助10
6秒前
年轻真好啊完成签到,获得积分10
6秒前
123发布了新的文献求助10
6秒前
领导范儿应助善良天抒采纳,获得10
6秒前
我刚上小学完成签到,获得积分10
7秒前
7秒前
好好努力小王完成签到,获得积分10
7秒前
7秒前
tao完成签到,获得积分10
8秒前
搬砖工人完成签到,获得积分10
8秒前
无心的平蝶完成签到,获得积分10
8秒前
9秒前
Orange应助Foliage采纳,获得10
9秒前
casey完成签到,获得积分10
10秒前
luluyang完成签到 ,获得积分10
10秒前
wxd完成签到,获得积分10
10秒前
水桶完成签到,获得积分10
11秒前
kangnakangna完成签到,获得积分10
11秒前
烟花应助aa采纳,获得10
11秒前
Eon完成签到 ,获得积分10
11秒前
俊秀的半雪完成签到,获得积分10
11秒前
11秒前
在水一方应助楠瓜采纳,获得10
11秒前
12秒前
岁岁菌完成签到,获得积分10
12秒前
Kamal完成签到,获得积分10
13秒前
忘久发布了新的文献求助20
13秒前
CucRuotThua完成签到,获得积分10
13秒前
热心烙完成签到,获得积分10
13秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
Cognitive Neuroscience: The Biology of the Mind 1000
Technical Brochure TB 814: LPIT applications in HV gas insulated switchgear 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
A Preliminary Study on Correlation Between Independent Components of Facial Thermal Images and Subjective Assessment of Chronic Stress 500
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3969033
求助须知:如何正确求助?哪些是违规求助? 3513900
关于积分的说明 11170818
捐赠科研通 3249256
什么是DOI,文献DOI怎么找? 1794708
邀请新用户注册赠送积分活动 875326
科研通“疑难数据库(出版商)”最低求助积分说明 804759