内质网
高尔基体
细胞生物学
内膜系统
分泌途径
信号
抗凋亡Ras信号级联
刺猬信号通路
信号转导
MAPK/ERK通路
生物
化学
作者
Vi K. Chiu,Trever G. Bivona,Angela Hach,J. Bernard Sajous,Joseph Silletti,Heidi Wiener,Ronald L. Johnson,Adrienne D. Cox,Mark R. Philips
摘要
Current models evoke the plasma membrane (PM) as the exclusive platform from which Ras regulates signalling. We developed a fluorescent probe that reports where and when Ras is activated in living cells. We show that oncogenic H-Ras and N-Ras engage Raf-1 on the Golgi and that endogenous Ras and unpalmitoylated H-Ras are activated in response to mitogens on the Golgi and endoplasmic reticulum (ER), respectively. We also demonstrate that H-Ras that is restricted to the ER can activate the Erk pathway and transform fibroblasts, and that Ras localized on different membrane compartments differentially engages various signalling pathways. Thus, Ras signalling is not limited to the PM, but also proceeds on the endomembrane.
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