Immuno-PCR: a promising ultrasensitive diagnostic method to detect antigens and antibodies

生物 计算生物学 抗原 还原(数学) 协议(科学) 计算机科学 免疫学 医学 替代医学 病理 几何学 数学
作者
Nada Malou,Didier Raoult
出处
期刊:Trends in Microbiology [Elsevier BV]
卷期号:19 (6): 295-302 被引量:106
标识
DOI:10.1016/j.tim.2011.03.004
摘要

Immuno-PCR (iPCR) is a method that combines the advantages of both enzyme-linked immunosorbent assay and PCR and is a powerful method for detecting low quantities of protein antigens. Despite its potential, for a long time iPCR was an underutilized method as evidenced by the low number of publications on its routine application. The introduction of ready-to-use reagents, the large choice in linker molecule, reduction of protocol time and the development of new systems is opening the way for iPCR to become a routine method for use as a microbial diagnostic. To understand how iPCR could become an indispensible microbial diagnostic, we review the evolution of iPCR, from its first classical format with numerous drawbacks to more sophisticated systems developed to circumvent these drawbacks. Immuno-PCR (iPCR) is a method that combines the advantages of both enzyme-linked immunosorbent assay and PCR and is a powerful method for detecting low quantities of protein antigens. Despite its potential, for a long time iPCR was an underutilized method as evidenced by the low number of publications on its routine application. The introduction of ready-to-use reagents, the large choice in linker molecule, reduction of protocol time and the development of new systems is opening the way for iPCR to become a routine method for use as a microbial diagnostic. To understand how iPCR could become an indispensible microbial diagnostic, we review the evolution of iPCR, from its first classical format with numerous drawbacks to more sophisticated systems developed to circumvent these drawbacks.
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