生物
分子生物学
互补DNA
北方斑点
肽序列
打开阅读框
基因
外显子
cDNA文库
基因簇
遗传学
作者
Irma Lemmens,Koen Kas,J. Merregaert,van de W J. M. Ven
出处
期刊:Genomics
[Elsevier]
日期:1998-05-01
卷期号:49 (3): 437-442
被引量:10
标识
DOI:10.1006/geno.1998.5296
摘要
In this report, the identification and molecular characterization of a novel gene, designatedTM7SF2,is reported. This gene was found in theFAUneighboring area (FAUNA) to which other genes have been mapped previously. The FAUNA gene cluster is located at chromosome 11q13 between landmarks H4B and D11S2196E. TheTM7SF2gene contains eight coding exons, and their splice site consensus sequences are consistent with the AG/GT rule. Northern blot analysis with a cDNA probe corresponding toTM7SF2revealed varying expression levels of a 1.7-kb transcript in adult human heart, brain, pancreas, lung, liver, skeletal muscle, kidney, ovary, prostate, and testis, but no detectable expression in placenta, spleen, thymus, small intestine, colon (mucosal lining), or peripheral blood leukocytes. The open reading frame in the cDNA sequence codes for a protein of 590 amino acids that is rich in glycine (23%) and arginine (17%) residues in its amino-terminal half and contains seven transmembrane domains in its carboxy-terminal half. The transmembrane region of the putative TM7SF2 protein shows amino acid sequence similarity to those of the lamin B receptor and the C14/C24 sterol reductase.
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