电穿孔
微电极
微流控
纳米技术
单细胞分析
电极
细胞
移液管
细胞培养
DNA
材料科学
实验室晶片
生物物理学
化学
生物
生物化学
基因
遗传学
物理化学
作者
Jessica Olofsson,Kerstin Nolkrantz,Frida Ryttsén,Bradley A. Lambie,Stephen G. Weber,Owe Orwar
标识
DOI:10.1016/s0958-1669(02)00003-4
摘要
Electroporation is a widely used method for the introduction of polar and charged agents such as dyes, drugs, DNA, RNA, proteins, peptides, and amino acids into cells. Traditionally, electroporation is performed with large electrodes in a batch mode for treatment of a large number of cells in suspension. Recently, microelectrodes that can produce extremely localized electric fields, such as solid carbon fiber microelectrodes, electrolyte-filled capillaries and micropipettes as well as chip-based microfabricated electrode arrays, have proven useful to electroporate single cells and subcellular structures. Single-cell electroporation opens up a new window of opportunities in manipulating the genetic, metabolic, and synthetic contents of single targeted cells in tissue slices, cell cultures, in microfluidic channels or at specific loci on a chip-based device.
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