内部核糖体进入位点
起始因子
真核翻译
生物
真核生物γ翻译起始因子4
五素未翻译区
真核起始因子
EIF4A1
翻译(生物学)
EIF4E公司
细胞生物学
核糖核酸
非翻译区
平动调节
遗传学
信使核糖核酸
基因
作者
Matthew J. Walker,Matthew D. Shortridge,Dreycey Albin,Lauren Y. Cominsky,Gabriele Varani
标识
DOI:10.1016/j.jmb.2020.01.001
摘要
Specialized translation initiation is a novel form of regulation of protein synthesis, whereby RNA structures within the 5′-UTR regulate translation rates of specific mRNAs. Similar to internal ribosome entry sites (IRESs), specialized translation initiation requires the recruitment of eukaryotic initiation factor 3 (eIF3), but also requires cap recognition by eIF3d, a new 5′-m7GTP recognizing protein. How these RNA structures mediate eIF3 recruitment to affect translation of specific mRNAs remains unclear. Here, we report the nuclear magnetic resonance (NMR) structure of a stem-loop within the c-JUN 5′ UTR recognized by eIF3 and essential for specialized translation initiation of this well-known oncogene. The structure exhibits similarity to eIF3 recognizing motifs found in hepatitis C virus (HCV)–like IRESs, suggesting mechanistic similarities. This work establishes the RNA structural features involved in c-JUN specialized translation initiation and provides a basis to search for small molecule inhibitors of aberrant expression of the proto-oncogenic c-JUN.
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