Facile transdermal delivery of upconversion nanoparticle by iontophoresis-responsive magneto-upconversion oleogel

Abstract The effective application of upconversion nanoparticle (UCNP) as a photo-medicine in skin cancers critically depends on a facile transdermal delivery process through topical route. Herein, combining two non-invasive techniques, i.e. skin permeation enhancement and alternating current (AC) induced iontophoresis, we report a controlled transdermal delivery of UCNP with a time advantage. We have synthesized a series of soybean oil-based oleogels termed as magneto-upconversion (MU) gels by incorporating a fixed amount of UCNP and different proportions of magnetic nanoparticle (MNP) using stearic acid-based gelator as a skin permeation enhancing agent. The microstructures of the synthesized MU gels were characterized by microscopy, X-Ray diffraction and vibrational spectroscopy. A detailed analysis of the electrical properties revealed a gradual increase in the electrical conductance in the MU gel series with increasing proportion of MNP. Such trend of conductance imparted proportional iontophoretic response within the respective MU gels, validated through the release of ciprofloxacin hydrochloride as a model drug preloaded within the oleogels. Through a series of skin permeation experiment using pig ear skin as animal model, we established that the UCNP was able to permeate the whole thickness of the skin within as little as 3 h, only when the two conditions, i.e. the presence of skin permeation enhancer and iontophoresis were met. Within the same time, UCNP permeation was enhanced by the presence of MNP in the MU gels upto 2 folds. Our study developed a rational method for the transdermal delivery of any electrically non-conducting nanoparticle in a faster and tunable way.