1‐Methylcyclopropene treatment controls ethanol accumulation associated with regulation of mitochondrial energy metabolism in kiwifruit ( Actinidia deliciosa ) cv. “Bruno” during storage at room temperature

Journal of Food BiochemistryVolume 44, Issue 7 e13273 FULL ARTICLE 1-Methylcyclopropene treatment controls ethanol accumulation associated with regulation of mitochondrial energy metabolism in kiwifruit (Actinidia deliciosa) cv. "Bruno" during storage at room temperature Maratab Ali, Maratab Ali orcid.org/0000-0003-0879-1233 School of Food Science and Biotechnology, Key Laboratory of Fruits and Vegetables Postharvest and Processing Technology Research of Zhejiang Province, Zhejiang Gongshang University, Hangzhou, P.R. ChinaSearch for more papers by this authorMuhammad Ammar Raza, Muhammad Ammar Raza School of Food Science and Biotechnology, Key Laboratory of Fruits and Vegetables Postharvest and Processing Technology Research of Zhejiang Province, Zhejiang Gongshang University, Hangzhou, P.R. ChinaSearch for more papers by this authorShenge Li, Shenge Li School of Food Science and Biotechnology, Key Laboratory of Fruits and Vegetables Postharvest and Processing Technology Research of Zhejiang Province, Zhejiang Gongshang University, Hangzhou, P.R. ChinaSearch for more papers by this authorChen Huan, Corresponding Author Chen Huan huanchen@zjgsu.edu.cn School of Food Science and Biotechnology, Key Laboratory of Fruits and Vegetables Postharvest and Processing Technology Research of Zhejiang Province, Zhejiang Gongshang University, Hangzhou, P.R. China Correspondence Chen Huan and Xiaolin Zheng, School of Food Science and Biotechnology, Key Laboratory of Fruits and Vegetables Postharvest and Processing Technology Research of Zhejiang Province, Zhejiang Gongshang University, Hangzhou 310018, P.R. China. Email: huanchen@zjgsu.edu.cn (C. H.), zheng9393@163.com (X. Z.)Search for more papers by this authorXiaolin Zheng, Corresponding Author Xiaolin Zheng zheng9393@163.com School of Food Science and Biotechnology, Key Laboratory of Fruits and Vegetables Postharvest and Processing Technology Research of Zhejiang Province, Zhejiang Gongshang University, Hangzhou, P.R. China Correspondence Chen Huan and Xiaolin Zheng, School of Food Science and Biotechnology, Key Laboratory of Fruits and Vegetables Postharvest and Processing Technology Research of Zhejiang Province, Zhejiang Gongshang University, Hangzhou 310018, P.R. China. Email: huanchen@zjgsu.edu.cn (C. H.), zheng9393@163.com (X. Z.)Search for more papers by this author Maratab Ali, Maratab Ali orcid.org/0000-0003-0879-1233 School of Food Science and Biotechnology, Key Laboratory of Fruits and Vegetables Postharvest and Processing Technology Research of Zhejiang Province, Zhejiang Gongshang University, Hangzhou, P.R. ChinaSearch for more papers by this authorMuhammad Ammar Raza, Muhammad Ammar Raza School of Food Science and Biotechnology, Key Laboratory of Fruits and Vegetables Postharvest and Processing Technology Research of Zhejiang Province, Zhejiang Gongshang University, Hangzhou, P.R. ChinaSearch for more papers by this authorShenge Li, Shenge Li School of Food Science and Biotechnology, Key Laboratory of Fruits and Vegetables Postharvest and Processing Technology Research of Zhejiang Province, Zhejiang Gongshang University, Hangzhou, P.R. ChinaSearch for more papers by this authorChen Huan, Corresponding Author Chen Huan huanchen@zjgsu.edu.cn School of Food Science and Biotechnology, Key Laboratory of Fruits and Vegetables Postharvest and Processing Technology Research of Zhejiang Province, Zhejiang Gongshang University, Hangzhou, P.R. China Correspondence Chen Huan and Xiaolin Zheng, School of Food Science and Biotechnology, Key Laboratory of Fruits and Vegetables Postharvest and Processing Technology Research of Zhejiang Province, Zhejiang Gongshang University, Hangzhou 310018, P.R. China. Email: huanchen@zjgsu.edu.cn (C. H.), zheng9393@163.com (X. Z.)Search for more papers by this authorXiaolin Zheng, Corresponding Author Xiaolin Zheng zheng9393@163.com School of Food Science and Biotechnology, Key Laboratory of Fruits and Vegetables Postharvest and Processing Technology Research of Zhejiang Province, Zhejiang Gongshang University, Hangzhou, P.R. China Correspondence Chen Huan and Xiaolin Zheng, School of Food Science and Biotechnology, Key Laboratory of Fruits and Vegetables Postharvest and Processing Technology Research of Zhejiang Province, Zhejiang Gongshang University, Hangzhou 310018, P.R. China. Email: huanchen@zjgsu.edu.cn (C. H.), zheng9393@163.com (X. Z.)Search for more papers by this author First published: 25 May 2020 https://doi.org/10.1111/jfbc.13273Citations: 5Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Abstract In this paper, the effects of 1-methylcyclopropene (1-MCP) treatment on the ethanol accumulation and mitochondrial energy metabolism in kiwifruit (Actinidia deliciosa) cv. "Bruno" were investigated during storage at room temperature (24 ± 1°C). The results showed that 1-MCP treatment significantly reduced the ethanol accumulation, maintained higher levels of adenosine triphosphate (ATP) content and energy charge (EC), enhanced enzymes activities involved in mitochondrial energy metabolism, such as succinic dehydrogenase, cytochrome C oxidase, H+-adenosine triphosphatase (H+-ATPase), and Ca2+-adenosine triphosphatase (Ca2+-ATPase), and regulated the NADH/NAD+ ratio, and pyruvate to tricarboxylic acid cycle (TCA) by suppressing the glutamate–pyruvate transaminase and promoting the pyruvate dehydrogenase activity, and decarboxylation of citric acid in harvested kiwifruit. These shifts in mitochondrial energy metabolism and oxidative phosphorylation correlated the higher ATP yield and an elevated EC with lower ethanol accumulation (13.71% of those in the control fruit) under 1-MCP treatment, and reduced the susceptibility of ethanol related off-flavor disorder in kiwifruit during storage. Practical applications A kiwifruit (Actinidia deliciosa) cv. "Bruno," a major cultivated kiwifruit in China, is prone to accumulate ethanol sharply after respiratory climacteric during storage. Ethanol has been accounted as a vital aroma volatile metabolite in various fruits. However, over accumulation of ethanol often leads to the development of alcohol off-flavor disorder, particularly in typical climacteric fruit such as kiwifruit. This work was aimed to maintain the flavor quality of kiwifruit cv. "Bruno" via improving the mitochondrial energy metabolism and functional TCA cycle, and limiting the ethanol accumulation under 1-MCP treatment. Thus, 1-MCP treatment could be beneficial to prevent the occurrence of alcohol off-flavor disorder along with sustainment of the flavor quality of kiwifruit cv. "Bruno" during storage at room temperature. CONFLICT OF INTEREST The authors have declared no conflicts of interest for this article. 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