动力素
细胞生物学
大肠杆菌
蛋白质表达
生物
化学
计算生物学
生物化学
细胞
内吞作用
基因
作者
Natalia V. Varlakhanova,M.G.J. Ford
出处
期刊:Methods in molecular biology
日期:2020-01-01
卷期号:: 17-27
标识
DOI:10.1007/978-1-0716-0676-6_2
摘要
The dynamin-related proteins (DRPs) are self-assembling membrane remodeling machines that are indispensable for fundamental cellular trafficking and homeostatic processes. We describe in this chapter protocols developed in our laboratory for purification of full-length and minimal constructs of Chaetomium thermophilum Vps1, the model fungal DRP, using mammalian and Escherichia coli expression systems.
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