适体
二茂铁
多巴胺
电极
化学
电化学
微电极
分析化学(期刊)
循环伏安法
材料科学
色谱法
物理化学
遗传学
生物
神经科学
作者
Hisham Abu-Ali,Cansu Özkaya,Frank Davis,Nik Walch,Alexei Nabok
出处
期刊:Chemosensors
[MDPI AG]
日期:2020-04-15
卷期号:8 (2): 28-28
被引量:20
标识
DOI:10.3390/chemosensors8020028
摘要
This work presents a proof of concept of a novel, simple, and sensitive method of detection of dopamine, a neurotransmitter within the human brain. We propose a simple electrochemical method for the detection of dopamine using a dopamine-specific aptamer labeled with an electrochemically active ferrocene tag. Aptamers immobilized on the surface of gold screen-printed gold electrodes via thiol groups can change their secondary structure by wrapping around the target molecule. As a result, the ferrocene labels move closer to the electrode surface and subsequently increase the electron transfer. The cyclic voltammograms and impedance spectra recorded on electrodes in buffer solutions containing different concentration of dopamine showed, respectively, the increase in both the anodic and cathodic currents and decrease in the double layer resistance upon increasing the concentration of dopamine from 0.1 to 10 nM L−1. The high affinity of aptamer-dopamine binding (KD ≈ 5 nM) was found by the analysis of the binding kinetics. The occurrence of aptamer-dopamine binding was directly confirmed with spectroscopic ellipsometry measurements.
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