中国仓鼠卵巢细胞
重组DNA
分子生物学
纤维蛋白原
生物
互补DNA
表达式向量
质粒
转染
亲和层析
生物化学
基因
受体
酶
作者
Susan T. Lord,C. G. Binnie,J. M. Hettasch,E. Strickland
出处
期刊:Blood Coagulation & Fibrinolysis
[Ovid Technologies (Wolters Kluwer)]
日期:1993-02-01
卷期号:4 (1): 55-59
被引量:18
标识
DOI:10.1097/00001721-199302000-00009
摘要
Our laboratory has been using protein engineering to study the relationship of primary structure to fibrinogen function. In order to examine genetically altered domains in the context of the intact, functional fibrinogen molecule, we have expressed recombinant human fibrinogen in Chinese Hamster Ovary (CHO) cells. The cDNA for each fibrinogen chain was individually cloned into the same expression vector. Each vector was cotransfected with the selection vector pRSVneo into CHO cells. In addition, the plasmids encoding Aα and γ were cotransfected with pRSVneo. Cells resistant to G418, a neomycin analogue, were isolated and clonal lines developed. Analysis of these lines demonstrated that CHO cells express and secrete free γ chain, and an Aα-γ complex. To obtain recombinant fibrinogen, the Aα-γ G418-resistant clones were transfected with the Bβ expression plasmid and a second selection vector, pMSVhis. Colonies resistant to neomycin and histidinol were selected and clonal lines obtained. These clones secreted biologically active recombinant human fibrinogen, which was purified from serum-free culture media by protamine-Sepharose chromatography. Analysis of the purified protein on SDS-polyacrylamide gels demonstrated a pattern indistinguishable from plasma fibrinogen. Removal of Asn-linked carbohydrate with glycosidase F revealed the presence of carbohydrate on the Bβ and γ chains, as is seen for plasma fibrinogen.
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