PI3K/AKT/mTOR通路
自噬
蛋白激酶B
细胞凋亡
化学
流式细胞术
间质细胞
活力测定
免疫印迹
运动性
细胞生长
子宫内膜异位症
癌症研究
细胞生物学
药理学
生物
医学
内科学
免疫学
生物化学
基因
作者
Liping Li,Qiwen Hu,Chen Jin,Xiumei Zhang,Chen Wang,Yong Luo
标识
DOI:10.1080/14786419.2023.2294113
摘要
Previous research has indicated that Polyphyllin I (PPI) possesses potent anticancer properties. However, its impact on endometriosis remains unexplored. This study aims to investigate the inhibitory effects of PPI on ectopic endometrial stromal cells (EESCs). The CCK-8 and flow cytometry results respectively showed that the cell viability of EESCs decreased and the number of apoptotic cells increased in a dosage dependent of PPI. Wound healing and transwell assays demonstrated a notable reduction in cell motility and migration ability in the PPI group. Moreover, the Western blot analysis revealed a decrease in p62 levels and an increase in LC3-II expression following PPI administration. Additionally, the protein levels of p-Akt and p-mTOR were observed to decrease with increasing concentrations of PPI, indicating the potential of PPI to induce autophagy in EESCs through modulation of the Akt/mTOR signalling pathway. Consequently, PPI holds promise as a targeted therapeutic agent for the management of endometriosis.
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