Gold nanoparticle-mediated fluorescence immunoassay for rapid and sensitive detection of Ochratoxin A

In this work, a fluorescence immunoassay based on horseradish peroxidase-labeled IgG (HRP-IgG)-modified gold nanoparticle (AuNP@HRP-IgG) probe was established for detection of ochratoxin A (OTA). Through the catalysis of HRP, the dopamine (DA) and 1,5-dihydroxynaphthalene (DHA) can rapidly generate azamonardine fluorescence compound (AFC) with intense yellow fluorescence. Large amounts of AFC can be formed within 4 min, which led to fluorescence enhancement at 545 nm. This new method displayed high sensitivity with a limit of detection (LOD) of 0.18 ng/mL and a linear range of 0.78-200 ng/mL for OTA. Meanwhile, the recoveries of OTA in corn samples were 101.41% - 113.45%. Due to the universality of the probe and the rapidity of signal output, the fluorescence immunoassay allowed rapid and sensitive detection of targets.