生物
前期
减数分裂
磷酸化
丝氨酸
细胞生物学
差速器(机械装置)
遗传学
基因
航空航天工程
工程类
作者
Hiroshi Kogo,Yuka Kikuchi-Kokubo,Yukiko Tajika,Akiko Iizuka‐Kogo,Hiroyasu Yamamoto,Maiko Ikezawa,Hiroki Kurahashi,Toshiyuki Matsuzaki
标识
DOI:10.1016/j.yexcr.2024.114133
摘要
Mouse HORMAD1 is a phospho-protein involved in multiple functions during meiotic prophase I. To obtain insight into the significance of its phosphorylation, we generated phospho-specific antibodies against two serine residues, Ser307 and Ser378, representing each of two serine clusters in mouse HORMAD1. The Ser307 phosphorylation is detectable from early leptotene substage in both wild-type and Spo11−/− spermatocytes, indicating that Ser307 is a primary and SPO11-independent phosphorylation site. In contrast, the Ser378 phosphorylation is negligible at earlier substages in wild-type and Spo11−/− spermatocytes. After mid-zygotene substage, the Ser378 phosphorylation is abundant on unsynapsed chromosome axes in wild-type spermatocytes and is detected only in a part of unsynapsed chromosome axes in Spo11−/− spermatocytes. We also generated a non-phosphorylated Ser307-specific antibody and found that Ser307 is phosphorylated on sex chromosome axes but is almost entirely unphosphorylated on desynapsed chromosome axes in diplotene spermatocytes. These results demonstrated a substage-specific phosphorylation status of mouse HORMAD1, which might be associated with multiple substage-specific functions.
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