circ‐ZEB1 Enhances NSCLC Metastasis and Proliferation by Modulating the miR‐491‐5p/EIF5A Axis

癌变 下调和上调 癌症研究 体内 荧光素酶 基因沉默 细胞生长 转移 小RNA 化学 癌症 生物 医学 内科学 转染 基因 生物化学 生物技术
作者
Qi Wang,Shengying Ling,Shaohua Xu,Lina Wu
出处
期刊:Analytical Cellular Pathology [Hindawi Limited]
卷期号:2025 (1)
标识
DOI:10.1155/ancp/5595692
摘要

Background: Circular RNAs (circRNAs), covalently closed single‐stranded RNAs, have been implicated in cancer progression. A previous investigation revealed that circ‐ZEB1 is expressed abnormally in liver cancer. However, the roles of circ‐ZEB1 in non‐small cell lung cancer (NSCLC) are unknown. Methods: In this study, we used fluorescence in situ hybridization (FISH) and RT‐qPCR to study circ‐ZEB1 expression in NSCLC cells and tissues. A luciferase reporter assay was performed to validate downstream targets of circ‐ZEB1. Transwell migration, 5‐ethynyl‐20‐deoxyuridine (EdU), and cell counting kit‐8 (CCK8) assays were performed to assess proliferation and migration. In vivo metastasis and tumorigenesis assays were also performed to investigate circ‐ZEB1 functions during NSCLC. Results: Our results showed that circ‐ZEB1 expression was increased in NSCLC tissues and cells. circ‐ZEB1 downregulation suppressed NSCLC cell proliferation as well as migration in vitro and in vivo. Luciferase data confirmed EIF5A and miR‐491‐5p as downstream targets of circ‐ZEB1. EIF5A overexpression and miR‐491‐5p suppression reversed NSCLC cell migration post circ‐ZEB1 silencing. Conclusion: Our collective findings advised that circ‐ZEB1 takes part in the malignant progression through regulating the miR‐491‐5p/EIF5A axis, highlighting its potential as an effective NSCLC therapeutic target.
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