等温滴定量热法
化学
量热法
滴定法
热力学
滴定曲线
水溶液
等温过程
物理化学
物理
作者
Dexing Li,Lan Chen,Ruimin Wang,Renxiao Liu,Guanglu Ge
标识
DOI:10.1021/acs.analchem.7b01091
摘要
Thermodynamic and kinetic signatures are pivotal information for revealing the binding mechanisms of biomolecules, and they play an indispensable role in drug discovery and optimization. While noncalorimetric methods measure only a part of these signatures, isothermal titration calorimetry (ITC) is considered to have the potential to acquire full signatures in an experiment. However, kinetic parameters are generally difficult to extract from ITC curves, as they are inevitably affected by the instrument-response function and the collateral heat of associated process during titrations. Thus, we herein report the development and validation of a full-curve-fitting method to resolve thermal power curves and to maximize the signal extraction using ITC. This method is then employed to quantify the dilution of an aqueous n-propanol solution and examine the inhibition of carbonic anhydrase by 4-carboxybenzenesulfonamide using a commercial instrument with a long apparent response time of ∼13 s.
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