化学
咬合
核化学
青霉胺
抗菌活性
组合化学
纳米颗粒
共轭体系
结合
亚硝酸盐
一氧化氮
纳米技术
有机化学
细菌
材料科学
数学
计算机科学
遗传学
硝酸盐
聚合物
生物
数学分析
计算机图形学(图像)
作者
Hamed Massoumi,R. Kumar,Manjyot Kaur Chug,Yun Qian,Elizabeth J. Brisbois
出处
期刊:ACS applied bio materials
[American Chemical Society]
日期:2022-04-20
卷期号:5 (5): 2285-2295
被引量:9
标识
DOI:10.1021/acsabm.2c00131
摘要
Therapeutic agents can be linked to nanoparticles to fortify their selectivity and targeted delivery while impeding systemic toxicity and efficacy loss. Titanium dioxide nanoparticles (TiNPs) owe their rise in biomedical sciences to their versatile applicability, although the lack of inherent antibacterial properties limits its application and necessitates the addition of bactericidal agents along with TiNPs. Structural modifications can improve TiNP’s antibacterial impact. The antibacterial efficacy of nitric oxide (NO) against a broad spectrum of bacterial strains is well established. For the first time, S-nitroso-N-acetylpenicillamine (SNAP), an NO donor molecule, was covalently immobilized on TiNPs to form the NO-releasing TiNP–SNAP nanoparticles. The TiNPs were silanized with 3-aminopropyl triethoxysilane, and N-acetyl-d-penicillamine was grafted to them via an amide bond. The nitrosation was carried out by t-butyl nitrite to conjugate the NO-rich SNAP moiety to the surface. The total NO immobilization was measured to be 127.55 ± 4.68 nmol mg–1 using the gold standard chemiluminescence NO analyzer. The NO payload can be released from the TiNP–SNAP under physiological conditions for up to 20 h. The TiNP–SNAP exhibited a concentration-dependent antimicrobial efficiency. At 5 mg mL–1, more than 99.99 and 99.70% reduction in viable Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli bacteria, respectively, were observed. No significant cytotoxicity was observed against 3T3 mouse fibroblast cells at all the test concentrations determined by the CCK-8 assay. TiNP–SNAP is a promising and versatile nanoparticle that can significantly impact the usage of TiNPs in a wide variety of applications, such as biomaterial coatings, tissue engineering scaffolds, or wound dressings.
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