癌变
食管癌
癌症研究
小RNA
抑制器
恶性肿瘤
癌症
基质凝胶
MTT法
医学
细胞生长
荧光素酶
生物
肿瘤科
病理
内科学
细胞培养
基因
转染
生物化学
遗传学
血管生成
作者
Geetika Suyal,Puja Pandey,Anoop Saraya,Rinu Sharma
标识
DOI:10.1016/j.yexmp.2021.104738
摘要
Esophageal cancer is an aggressive malignancy. miR-335-5p is reported to possess both tumour suppressor and tumour promoter activities in different cancers.We investigated the role of miR-335-5p in esophageal cancer by expression and functional studies.The role of miR-335-5p in ESCC was evaluated using MTT assay, cell cycle analysis, colony formation assay, scratch assay, matrigel invasion, and migration assay.Our expression studies showed a significantly decreased expression of tissue and circulating miR-335-5p in esophageal cancer. Our results herein report a key tumour suppressive role of miR-335-5p in esophageal carcinogenesis by inhibiting proliferation, migration, and invasion in ESCC cells. Using RNA-seq and Insilico analysis we found TTK to be a newly identified direct target and confirmed it by using luciferase assay.Overall, our expression and functional analysis results demonstrated herein point towards the potential role of miR-335-5p in esophageal tumorigenesis. Moreover, this is the first report showing TTK as a downstream target of miR-335-5p.
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