化学
杨梅素
芦丁
DPPH
槲皮素
脂质体
类黄酮
莫林
紫杉醇
脂质双层
非西汀
生物化学
膜
抗氧化剂
山奈酚
医学
病理
作者
Anil K. Ratty,Junzo Sunamoto,N.P. Das
标识
DOI:10.1016/0006-2952(88)90499-6
摘要
The interaction of the antiperoxidative flavonoids namely, quercetin, quercetrin, rutin, myricetin, phloretin, phloridzin, catechin, morin and taxifolin with the 1,1,-diphenyl-2-plcrylhydrazyl (DPPH) free radical was demonstrated. Flavonoid-DPPH interaction was looked at in the absence and presence of liposomes so as to reveal some information on bilayers. Perturbations in the lipid bilayers were monitored with the fluorescent probe, dansylhexadecylamine (DSHA). It was observed that the interaction of the flavonoids on the lipid bilayer occurred in the polar zone of the lipid bilayers. The flavonoids were able to scavenge free radicals and could do so in biomembranes. It is suggested that the DPPH free radical abstracts the phenolic hydrogen of the flavonoid molecule and that this could be the general mechanism of the scavenging action of the antiperoxidative flavonoids. The effects of the flavonoids on soybean lipoxygenase-1 were investigated both in buffer and also in liposomal suspension. All the flavonoids studied showed inhibition of the enzyme in both systems but the inhibition was greater in the liposomal suspension. Quercetin was the most potent and it inhibited the lipoxygenase in the liposomal suspension by about 42% while the other flavonoids inhibited the enzyme by about 14–23%. We observed that the effect of myricetin and quercetin on the enzyme was pH dependent.
科研通智能强力驱动
Strongly Powered by AbleSci AI