Tannic Acid Cross-linked Collagen Scaffolds and Their Anti-cancer Potential in a Tissue Engineered Breast Implant

单宁酸 间充质干细胞 化学 脂肪生成 生物化学 间质细胞 生物物理学 癌细胞 细胞 体外 细胞生物学 生物医学工程 癌症 癌症研究 生物 有机化学 医学 遗传学
作者
Cheryl Cass,Karen J. L. Burg
出处
期刊:Journal of Biomaterials Science-polymer Edition [Informa]
卷期号:23 (1-4): 281-298 被引量:55
标识
DOI:10.1163/092050610x550331
摘要

Tannic acid (TA) is a hydrolysable plant tannin, and it has been determined that TA functions as a collagen cross-linking agent through hydrogen-bonding mechanisms and hydrophobic effects. Since TA may have anti-tumor properties, it may be a viable cross-linking agent for collagen-based breast tissue scaffolds. The goal of this work was to determine if TA cross-linked scaffolds induce apoptotic processes in MCF-7 cancer cells, with minimal toxic effect on healthy D1 mesenchymal stem-like stromal cells. Cross-linked collagen scaffolds that were uniform, easily reproduced, easily characterized, and readily used in cell culture were manufactured. Thermal denaturation temperatures of the cross-linked scaffolds (68°C) were shown to be significantly higher when compared to those of uncross-linked scaffolds (55°C). Scanning electron microscopy images demonstrated the replacement of irregular collagen fibers with sheet-like structures upon cross-linking. The cross-linking solution concentration of TA that appears to be best for inducing apoptotic processes in MCF-7 cells, while minimizing toxic effect on D1 cells, is 1 mg/ml. At this concentration, the MCF-7 cell metabolic activity did not change over a 72-h period (i.e., proliferation was limited) while there was an increase in metabolic activity of D1 cells over the 72-h period. TA did appear to inhibit the production of lipid by D1 cells cultured in an adipogenic cocktail; in the future, the rate and duration of inhibition could be tailored to allow gradual bulking of the implant. The results suggest that the level of TA cross-linking can be modulated to provide optimal use in a tissue engineering composite.
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