Receptor kinases with leucine‐rich repeats are enriched in Triton X‐100 insoluble plasma membrane microdomains from plants

In mammals, signalling components at the cell surface are clustered in Triton X‐100 insoluble plasma membrane microdomains. We isolated plasma membrane microdomains from Arabidopsis and mustard cotyledons and determined their protein composition by mass spectrometry. Although the protein composition of the plant vesicles differ from the composition of the animal vesicles, they are also enriched in signalling components. We identified at least seven receptor kinases with leucine‐rich repeats, 10 other kinases, the β subunit of heterotrimeric G‐proteins and five small GTP‐binding proteins. Thus, specific signalling components are highly enriched in plant plasma membrane microdomains while others are excluded.