乳酸乳球菌
绿色荧光蛋白
质粒
分子生物学
转染
穿梭机载体
HEK 293细胞
互补DNA
荧光显微镜
生物
基因传递
DNA
基因
化学
细菌
荧光
重组DNA
生物化学
遗传学
载体(分子生物学)
物理
量子力学
乳酸
作者
Yijie Li,Huan‐Huan Liu,Fuchun Zhang
出处
期刊:PubMed
日期:2012-12-01
卷期号:28 (12): 1328-30
被引量:1
摘要
To develop a co-delivery system of exogenous protein and DNA into mammalian cells using Lactococcus lactis (L. lactis).We constructed E.coli-L.lactis shuttle plasmid, pMG36e-RFP/eGFP, containing a L.lactis expression cassette with the cDNA of red fluorescent protein (RFP) gene and a eukaryotic expression cassette with the cDNA of enhanced green fluorescent protein (eGFP), then electrotransformated it into L.lactis cells, and co-cultured with 293T cells to evaluate the role of the system in the improvement of gene delivering efficiency after L.lactis cells were pretreated with glycine. RFP and GFP expressed in 293T cells was monitored by fluorescence microscopy.Fluorescence microscopy revealed that the RFP and GFP was expressed in 293T cells.The co-delivery system of protein and DNA into mammalian cells using L.lactis was constructed successfully.
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