Interactive effects of molecular weight and degree of substitution on biological activities of arabinoxylan and its hydrolysates from triticale bran

Arabinoxylan (AX) has many beneficial health effects that are closely related to its structural characteristics. The current study aimed to investigate the effects of molecular weight (Mw) and degree of substitution (DS) on the antioxidant and hypoglycemic activities of triticale bran AX and its hydrolysates in vitro. At low and similar Mw, the antioxidant activity of AX was inversely proportional to its DS. When DS was close, the antioxidant activity of AX was inversely proportional to its Mw at high DS, but the opposite result was found at low DS. As for the hypoglycemic performance, when DS was similar, the hypoglycemic activity of AX was proportional to its Mw. At low and similar Mw, the α-glucosidase inhibitory ability and glucose adsorption ability of AX was positively correlated with DS, whereas the α-amylase inhibitory ability and glucose delayed absorption ability showed the opposite results. Mw and DS had significant effects on the antioxidant and hypoglycemic activities of AX, and these two factors often need to be combined to explain the varied effects under different conditions. • Bioactivities of AX were closely correlated to their Mw and DS. • At low and similar Mw, the antioxidant activity of AX was inversely proportional to its DS. • At similar DS, the antioxidant activity of AX was inversely proportional to its Mw at high DS, but opposite at low DS. • At similar DS, the hypoglycemic activity of AX was directly proportional to its Mw.