Osteogenetic effect of the compound of calcium citrate and recombinant human bone morphogenetic protein

Objective To study the osteogenetic effect of the compound of calcium citrate and recombinant human bone morphogenetic protein(rhBMP).Methods Fifteen male Kunming mice,2months of age,were used to obtain the optimal composition ratio of calcium citrate and rhBMP in an in vitro experiment.A compound of 10 mg of calcium citrate plus 2 mg of rhBMP was implanted into the mouse thigh muscle pouch in the experimental group while in the control group BMP alone was implanted.Samples were harvested at 2,4,6 weeks postoperation for gross and histological observations.The areas and densities of new bone formation in both groups were counted.Results At all time points,the new bone areas were significantly larger and the new bone densities were significantly greater in the experimental group than in the control group ( P ＜ 0.05).In the experimental group,more mature trabecular bone appeared at 2 weeks,with higher destiny and more proliferated and differentiated osteoblasts.At 4 weeks,there was a larger area of newly formed pink bone and larger bone trabeculae.At 6 weeks,vascularization appeared and medullary cavities formed with increased bone destiny.Conclusion Since the compound of calcium citrate and th-BMP has a fine effect on osteoinduction and osteogenesis,it can be used as a new compound artificial bone to repair bone defects. Key words: Calcium citrate;  Bone morphogenetic protein;  Osteoblasts