化学发光
体内
赫拉
免疫疗法
材料科学
生物物理学
癌症
费斯特共振能量转移
荧光
化学
癌症研究
光化学
体外
生物化学
生物
物理
量子力学
生物技术
有机化学
遗传学
作者
Dong Cui,Jingchao Li,Xuhui Zhao,Kanyi Pu,Ruiping Zhang
标识
DOI:10.1002/adma.201906314
摘要
Abstract Real‐time in vivo imaging of immunoactivation is critical for longitudinal evaluation of cancer immunotherapy, which, however, is rarely demonstrated. This study reports semiconducting polymer nanoreporters (SPNRs) with superoxide anion (O 2 •− )‐activatable chemiluminescence signals for in vivo imaging of immunoactivation during cancer immunotherapy. SPNRs are designed to comprise an SP and a caged chemiluminescence phenoxy‐dioxetane substrate, which respectively serve as the chemiluminescence acceptor and donor to enable intraparticle chemiluminescence resonance energy transfer. SPNRs are intrinsically fluorescent but only become chemiluminescent upon activation by O 2 •− . Representing the first O 2 •− ‐activatable near‐infrared chemiluminescent reporter, SPNR3 sensitively differentiates higher O 2 •− levels in immune cells from other cells including cancer and normal cells. Following systemic administration, SPNR3 passively accumulates into tumors in living mice and activates the chemiluminescence signals responding to the concentration of O 2 •− in the tumor microenvironment. Moreover, the enhancement of in vivo chemiluminescence signal after cancer immunotherapy is correlated with increased population of T cells in the tumor, proving its feasibility in tracking of T cell activation. Thus, SPNRs represent the first kind of chemiluminescent reporters competent for in vivo imaging of immunoactivation.
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