机械敏感通道
生物
机械转化
形态发生
斑马鱼
细胞生物学
心脏发育
细胞外基质
细胞
细胞外
心脏瓣膜
机械生物学
心肌细胞
解剖
表型
心脏瓣膜
胚胎心脏
离子通道
生物化学
受体
基因
作者
Hélène Vignes,Christina Vagena-Pantoula,Mangal Prakash,Hajime Fukui,Caren Norden,Naoki Mochizuki,Florian Jug,Julien Vermot
标识
DOI:10.1016/j.devcel.2022.02.011
摘要
Organ morphogenesis involves dynamic changes of tissue properties while cells adapt to their mechanical environment through mechanosensitive pathways. How mechanical cues influence cell behaviors during morphogenesis remains unclear. Here, we studied the formation of the zebrafish atrioventricular canal (AVC) where cardiac valves develop. We show that the AVC forms within a zone of tissue convergence associated with the increased activation of the actomyosin meshwork and cell-orientation changes. We demonstrate that tissue convergence occurs with a reduction of cell volume triggered by mechanical forces and the mechanosensitive channel TRPP2/TRPV4. Finally, we show that the extracellular matrix component hyaluronic acid controls cell volume changes. Together, our data suggest that multiple force-sensitive signaling pathways converge to modulate cell volume. We conclude that cell volume reduction is a key cellular feature activated by mechanotransduction during cardiovascular morphogenesis. This work further identifies how mechanical forces and extracellular matrix influence tissue remodeling in developing organs.
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