阿霉素
衰老
体内
脂褐素
药理学
癌症研究
生物
医学
内科学
化疗
细胞生物学
生物技术
作者
Tianyue Sun,Lanxin Zhang,Jiali Feng,Lingyuan Bao,Jiqun Wang,Zhouzhi Song,Zhifan Mao,Jian Li,Zelan Hu
标识
DOI:10.1016/j.exger.2022.111800
摘要
With a rise in the need to develop anti-aging drugs, a growing number of in vivo studies evaluating the efficacy of potential drug candidates have used doxorubicin-induced aging mice. However, changes in the biomarkers of senescent cells have not been reported in detail in these animals. To lay a foundation for the use of doxorubicin-induced aging mice, we examined the biomarkers of hepatic and renal senescent cells in these mice. We found that the 5 mg/kg doxorubicin dose is optimal to induce cellular senescence in mice. Subsequently, using this dose, we found that doxorubicin-induced an increase in senescence-associated β-galactosidase (SA-β-gal) positive cells in the kidney and lipofuscin accumulation in the liver. Some markers of senescent cells (p21WAF1/CIP1, p16INK4A, and γH2AX) were also significantly upregulated by doxorubicin and then counteracted by metformin treatment. These preliminary findings support the application of doxorubicin-induced aging mice as an animal model to evaluate the efficacy of anti-aging drug candidates.
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