N-acetylcysteine potentiates the tumor cytotoxicity of cytokine-induced killer cells

细胞因子诱导的杀伤细胞 细胞毒性T细胞 颗粒酶 颗粒酶B 人口 细胞毒性 穿孔素 生物 细胞因子 癌症研究 细胞溶解 流式细胞术 细胞培养 K562细胞 免疫学 CD3型 T细胞 免疫系统 CD8型 医学 体外 生物化学 白血病 遗传学 环境卫生
作者
Pornpimon Ek-Eudomsuk,Chonvara Chalermrujinanant,Kitipong Soontrapa
出处
期刊:Asian Pacific Journal of Allergy and Immunology [Allergy and Immunology Society of Thailand]
标识
DOI:10.12932/ap-280921-1245
摘要

Cytokine-induced killer (CIK) cells are an ex vivo expanded heterogeneous population of natural killer (NK)-like T cells that can exert potent MHC-unrestricted antitumor activity. A number of pre-clinical and clinical studies have demonstrated that CIK cells can serve as a safe and potent immunotherapy of malignant tumors. N-acetylcysteine (NAC) has been demonstrated to enhance the T-cell functions by increasing their proliferation and cytokine production.To investigate whether the incorporation of NAC to CIK cell culture could enhance the antitumor activity of CIK cells.The phenotypes of human CIK cells, including CD3+CD56+, IFN-γ, granzyme B, and perforin, were determined by flow cytometry. The cytotoxic activity against the human erythroleukemic cell line (K562) and cholangiocarcinoma cell line (CL6) prelabeled with CFSE was investigated by flow cytometry. The mRNA expression levels of IFNG, PRF1, and GZMB were measured by real-time PCR.By adding NAC into CIK cell culture, the percentage of CD3+CD56+ cells along with the expression of Th1 cytokines and cytolytic granules increased significantly, resulting in an improvement of cytotoxicity against the cancer cell lines CL6 and K562.The incorporation of NAC into CIK culture can markedly improve the cytotoxicity against cancer cells due to the significant increase in the major effector population of CIK cells expressing Th1 cytokines and cytolytic granules.
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