Self-emulsifying System Co-loaded with Paclitaxel and Coix Seed Oil Deeply Penetrated to Enhance Efficacy in Cervical Cancer

紫杉醇 赫拉 微乳液 化学 MTT法 维加维斯 溶解度 细胞毒性 色谱法 核化学 体外 有机化学 医学 化疗 生物化学 外科 病理 中医药 替代医学 肺表面活性物质
作者
Yunyan Chen,Shaozhen Wang,Qiyan Hu,Lingyun Zhou
出处
期刊:Current Drug Delivery [Bentham Science]
卷期号:20 (7): 919-926 被引量:5
标识
DOI:10.2174/1567201819666220628094239
摘要

Paclitaxel (PTX), voted as the promising natural medicine molecule, is widely used in the treatment of cancers. Nevertheless, its clinical application is strictly limited by its poor water solubility.CP-MEs (Paclitaxel-coix seed oil coloaded microemulsion), a small-sized self-emulsifying nanoemulsion formed from a combination of PTX and coix seed oil (CSO), was developed in order to improve the solubility of paclitaxel and enhance anti-cervical cancer efficacy in vitro. CSO was selected as the oil phase to replace conventional organic solvents and achieve a synergistic anti-tumor effect with paclitaxel.Pseudoternary phase diagram was applied to the study of CP-MEs formulation. CP-MEs were prepared and characterized by transmission electron microscopy (TEM) and dynamic light scattering (DLS). The encapsulation efficiency and drug loading efficiency (EE and LE) were detected by HPLC. MTT was adopted to evaluate the cytotoxicity of CP-MEs against HeLa cells. The cellular uptake and apoptotic ratio of CP-MEs were evaluated by flow cytometry. Notably, HeLa 3D tumor spheroid was adopted to evaluate tumor permeability of different size microemulsions as the model.The best self-emulsifying ability was exhibited by HS 15: PEG 400 combination. The appearance of CP-MEs was clear and transparent, which exhibited a small size (30.28 ± 0.36) and a slight negative surface charge (-4.40 ± 1.13) mV. The EE and LE of CP-MEs were 98.80% and 0.978%, respectively. The cumulative release rate within 48 h of the CP-MEs was 80.21%. In cellular studies, the uptake of fluorescein isothiocyanate (FITC) labeled CP-MEs (FITC/C-MEs) was 17.86-fold higher than the free FITC group, leading to significant synergistic anticancer activity in terms of cytotoxicity and apoptosis induction in vitro. The apoptotic rate of CP-MEs treated was 1.70-fold higher than PTXtreated. Notably, the penetration of CP-MEs in the HeLa 3D tumor sphere model was enhanced, which was related to deeply penetrated microemulsion of small size mediated at the tumor site.With the advantage of the small-sized self-emulsifying system, CP-MEs hold great potential to become an efficient nano drug delivery system for cervical cancer treatment in the clinic.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI

祝大家在新的一年里科研腾飞
科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
曾sir发布了新的文献求助10
1秒前
Momo发布了新的文献求助30
1秒前
1秒前
冰淇淋完成签到,获得积分10
4秒前
qyq完成签到,获得积分10
5秒前
5秒前
fan完成签到,获得积分10
6秒前
6秒前
guo_a_n完成签到,获得积分10
6秒前
wdl完成签到 ,获得积分10
6秒前
周小凡发布了新的文献求助10
7秒前
碧蓝的硬币完成签到,获得积分20
7秒前
8秒前
科研通AI2S应助mara采纳,获得10
8秒前
dcdc发布了新的文献求助10
8秒前
牛乃糖完成签到 ,获得积分10
9秒前
汉堡包应助雨点采纳,获得10
9秒前
9秒前
9秒前
10秒前
含糊的灵雁完成签到,获得积分10
10秒前
11秒前
苏落白的白完成签到,获得积分10
11秒前
lala完成签到 ,获得积分10
11秒前
11秒前
情怀应助雨无意采纳,获得10
11秒前
Emma完成签到,获得积分10
11秒前
Molly应助仁爱的尔蓝采纳,获得10
13秒前
zys完成签到,获得积分10
13秒前
清新的万天完成签到,获得积分10
13秒前
14秒前
墨1234发布了新的文献求助30
14秒前
P_L发布了新的文献求助10
15秒前
CodeCraft应助hnwsz采纳,获得10
16秒前
x小牛马来咯关注了科研通微信公众号
18秒前
19秒前
海风完成签到,获得积分10
20秒前
二傻完成签到 ,获得积分10
20秒前
20秒前
21秒前
高分求助中
Востребованный временем 2500
The Three Stars Each: The Astrolabes and Related Texts 1500
Classics in Total Synthesis IV: New Targets, Strategies, Methods 1000
Les Mantodea de Guyane 800
Mantids of the euro-mediterranean area 700
The Oxford Handbook of Educational Psychology 600
有EBL数据库的大佬进 Matrix Mathematics 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 内科学 物理 纳米技术 计算机科学 遗传学 化学工程 基因 复合材料 免疫学 物理化学 细胞生物学 催化作用 病理
热门帖子
关注 科研通微信公众号,转发送积分 3414243
求助须知:如何正确求助?哪些是违规求助? 3016416
关于积分的说明 8876262
捐赠科研通 2704183
什么是DOI,文献DOI怎么找? 1482594
科研通“疑难数据库(出版商)”最低求助积分说明 685454
邀请新用户注册赠送积分活动 680191