免疫沉淀
染色质免疫沉淀
核糖核酸
染色质
细胞生物学
RNA结合蛋白
长非编码RNA
生物
非编码RNA
分子生物学
计算生物学
基因表达
基因
生物化学
发起人
出处
期刊:Methods in molecular biology
日期:2017-10-13
卷期号:: 241-250
被引量:48
标识
DOI:10.1007/978-1-4939-7371-2_17
摘要
Long noncoding RNAs (lncRNAs) have important roles in shaping chromatin by targeting chromatin-modifying enzymes to distinct genomic sites. This section covers two methods to analyze lncRNA-protein interactions. The RNA-protein pull-down assays use either bead-bound proteins to capture in vitro transcripts, or immobilized synthetic RNAs to bind proteins from cell lysates. In the RNA immunoprecipitation (RIP) assay, endogenous RNAs are co-immunoprecipitated with a protein of interest. Both the methods can be applied to material from proliferating and quiescent cells, thus providing insights into how lncRNA-protein interactions are altered between these two cellular states.
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