Negatively Charged Carbon Dots Employed Symplastic and Apoplastic Pathways to Enable Better Plant Delivery than Positively Charged Carbon Dots

碳纤维 质外体 材料科学 纳米技术 细胞外 染色体易位 细胞内 荧光 生物物理学 化学 纳米颗粒 复合材料 生物 生物化学 细胞壁 复合数 物理 量子力学 基因
作者
Linlin Chen,Lan Zhu,Huiling Cheng,Wenying Xu,Guangjing Li,Yuqing Zhang,Jiangjiang Gu,Lu Chen,Zhouli Xie,Zhaohu Li,Honghong Wu
出处
期刊:ACS Nano [American Chemical Society]
标识
DOI:10.1021/acsnano.4c05362
摘要

Efficient delivery of nanoparticles (NPs) to plants is important for agricultural application. However, to date, we still lack knowledge about how NPs' charge matters for its translocation pathway, i.e., symplastic and apoplastic pathways, in plants. In this study, we synthesized and used negatively charged citrate sourced carbon dots (C-CDs, −37.97 ± 1.89 mV), Cy5 coated C-CDs (Cy5-C-CDs, −41.90 ± 2.55 mV), positively charged PEI coated carbon dots (P-CDs, +43.03 ± 1.71 mV), and Cy5 coated P-CDs (Cy5-P-CDs, +48.80 ± 1.21 mV) to investigate the role of surface charges and coatings on the employed translocation pathways (symplastic and apoplastic pathways) of charged NPs in plants. Our results showed that, different from the higher fluorescence intensity of P-CDs and Cy5-P-CDs in extracellular than intracellular space, the fluorescence intensity of C-CDs and Cy5-C-CDs was similar between intracellular and extracellular space in cucumber and cotton roots. It suggests that the negatively charged CDs were translocated via both symplastic and apoplastic pathways, but the positively charged CDs were mainly translocated via the apoplastic pathway. Furthermore, our results showed that root applied negatively charged C-CDs demonstrated higher leaf fluorescence than did positively charged P-CDs in both cucumber (8.09 ± 0.99 vs 3.75 ± 0.23) and cotton (7.27 ± 1.06 vs 3.23 ± 0.22), indicating that negatively charged CDs have a higher translocation efficiency from root to leaf than do positively charged CDs. It should be noted that CDs do not affect root cell activities, ROS level, and photosynthetic performance in cucumber and cotton, showing its good biocompatibility. Overall, this study not only figured out that root applied negatively charged CDs employed both symplastic and apoplastic pathways to do the transportation in roots compared with mainly the employment of apoplastic pathway for positively charge CDs, but also found that negatively charge CDs could be more efficiently translocated from root to leaf than positively charged CDs, indicating that imparting negative charge to NPs, at least CDs, matters for its efficient delivery in crops.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
陈军应助学术小白采纳,获得10
1秒前
彭于晏完成签到,获得积分10
1秒前
科研通AI2S应助lennon962464采纳,获得10
1秒前
1秒前
念与惜完成签到,获得积分10
1秒前
星辰大海应助阳佟听荷采纳,获得10
2秒前
Billy发布了新的文献求助10
2秒前
贵金属完成签到,获得积分10
3秒前
fasfsbbsdd完成签到,获得积分20
3秒前
顺心的莫茗完成签到,获得积分10
4秒前
4秒前
开开发布了新的文献求助10
4秒前
4秒前
5秒前
5秒前
linty应助洋芋片采纳,获得10
5秒前
我是老大应助十三采纳,获得10
5秒前
5秒前
6秒前
科研发布了新的文献求助10
6秒前
李哈完成签到,获得积分10
7秒前
小马想毕业完成签到,获得积分10
7秒前
7秒前
7秒前
研友_VZG7GZ应助哈哈哈哈哈采纳,获得10
7秒前
小心翼翼完成签到,获得积分10
8秒前
8秒前
wanci应助fasfsbbsdd采纳,获得10
8秒前
Lucas应助莫莫采纳,获得10
9秒前
tommorw发布了新的文献求助10
10秒前
JamesPei应助可以的采纳,获得10
10秒前
盆浴烟发布了新的文献求助10
10秒前
金金金完成签到,获得积分10
10秒前
QMM完成签到 ,获得积分10
11秒前
bkagyin应助nibaba采纳,获得30
11秒前
David发布了新的文献求助10
11秒前
12秒前
小心翼翼发布了新的文献求助10
12秒前
天边的云发布了新的文献求助10
12秒前
12秒前
高分求助中
Evolution 10000
Sustainability in Tides Chemistry 2800
The Young builders of New china : the visit of the delegation of the WFDY to the Chinese People's Republic 1000
юрские динозавры восточного забайкалья 800
Diagnostic immunohistochemistry : theranostic and genomic applications 6th Edition 500
Chen Hansheng: China’s Last Romantic Revolutionary 500
China's Relations With Japan 1945-83: The Role of Liao Chengzhi 400
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3148466
求助须知:如何正确求助?哪些是违规求助? 2799588
关于积分的说明 7836005
捐赠科研通 2456991
什么是DOI,文献DOI怎么找? 1307679
科研通“疑难数据库(出版商)”最低求助积分说明 628245
版权声明 601655