生物
磷酸化
真菌
病原真菌
蛋白质组学
激酶
调节器
细胞生物学
计算生物学
植物
遗传学
基因
作者
Neftaly Cruz-Mireles,Míriam Osés-Ruiz,Paul Derbyshire,Clara Jégousse,Lauren S. Ryder,Mark Jave A. Bautista,Alice Bisola Eseola,Jan Sklenář,Bozeng Tang,Yan Xia,Weibin Ma,Kim Findlay,Vincent Were,Dan MacLean,Nicholas J. Talbot,Frank L.H. Menke
标识
DOI:10.1101/2023.08.19.553964
摘要
SUMMARY Many of the world’s most devastating crop diseases are caused by fungal pathogens which elaborate specialized infection structures to invade plant tissue. Here we present a quantitative mass spectrometry-based phosphoproteomic analysis of infection-related development by the rice blast fungus Magnaporthe oryzae , which threatens global food security. We mapped 8,005 phosphosites on 2,062 fungal proteins, revealing major re-wiring of phosphorylation-based signaling cascades during fungal infection. Comparing phosphosite conservation across 41 fungal species reveals phosphorylation signatures specifically associated with biotrophic and hemibiotrophic fungal infection. We then used parallel reaction monitoring to identify phosphoproteins directly regulated by the Pmk1 MAP kinase that controls plant infection by M. oryzae . We define 33 substrates of Pmk1 and show that Pmk1-dependent phosphorylation of a newly identified regulator, Vts1, is required for rice blast disease. Defining the phosphorylation landscape of infection therefore identifies potential therapeutic interventions for control of plant diseases.
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