光敏剂
紧身衣
光动力疗法
化学
单线态氧
体内
体外
部分
衰老
细胞生物学
生物物理学
癌症研究
荧光
生物化学
生物
光化学
氧气
立体化学
物理
有机化学
生物技术
量子力学
作者
Jacky C. H. Chu,Blanca Escriche‐Navarro,Junlong Xiong,Alba García‐Fernández,Ramón Martínez‐Máñez,Dennis K. P. Ng
标识
DOI:10.1002/advs.202401012
摘要
Abstract Senescence is a cellular response having physiological and reparative functions to preserve tissue homeostasis and suppress tumor growth. However, the accumulation of senescent cells would cause deleterious effects that lead to age‐related dysfunctions and cancer progression. Hence, selective detection and elimination of senescent cells are crucial yet remain a challenge. A β‐galactosidase (β‐gal)‐activated boron dipyrromethene (BODIPY)‐based photosensitizer (compound 1 ) is reported here that can selectively detect and eradicate senescent cells. It contains a galactose moiety connected to a pyridinium BODIPY via a self‐immolative nitrophenylene linker, of which the photoactivity is effectively quenched. Upon interactions with the senescence‐associated β‐gal, it undergoes enzymatic hydrolysis followed by self‐immolation, leading to the release of an activated BODIPY moiety by which the fluorescence emission and singlet oxygen generation are restored. The ability of 1 to detect and eliminate senescent cells is demonstrated in vitro and in vivo, using SK‐Mel‐103 tumor‐bearing mice treated with senescence‐inducing therapy. The results demonstrate that 1 can be selectively activated in senescent cells to trigger a robust senolytic effect upon irradiation. This study breaks new ground in the design and application of new senolytic agents based on photodynamic therapy.
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