A dual-functional fluorescence probe for simultaneous in vivo imaging of Aβ aggregates and hydrogen peroxide in the brain of mice with Alzheimer's disease

Open AccessCCS ChemistryRESEARCH ARTICLES2 Jan 2025A dual-functional fluorescence probe for simultaneous in vivo imaging of Aβ aggregates and hydrogen peroxide in the brain of mice with Alzheimer's disease Jiajia Lv, Hongyu Li, Jie Gao, Nan Dong, Wen Shi, Huimin Ma and Zeli Yuan Jiajia Lv , Hongyu Li , Jie Gao , Nan Dong , Wen Shi , Huimin Ma and Zeli Yuan https://doi.org/10.31635/ccschem.025.202404927 SectionsSupplemental MaterialAboutPDF ToolsAdd to favoritesDownload CitationsTrack Citations ShareFacebookTwitterLinked InEmail Amyloid-β (Aβ) plaques and reactive oxygen species are two important and highly correlated pathological markers of Alzheimer's disease (AD). Therefore, the simultaneous reliable detection of the two markers is essential for elucidating their pathological roles in AD. In this study, a dual-functional fluorescence probe (NPBZ) was developed for simultaneous in vivo imaging of Aβ plaques and hydrogen peroxide (H2O2). NPBZ, composed of a rotatable D-π-A structural fluorophore and a H2O2-responsive p-pinacolborylbenzyl group, showed a large fluorescence increase at 708 nm upon interaction with Aβ aggregates; however, after reacting with H2O2, the emission of NPBZ was blue-shifted to 618 nm. The large spectral shift (90 nm) enabled the independent recognition of H2O2 and Aβ aggregates in two channels, thus achieving the dual-functional detection. Moreover, NPBZ can target mitochondria and monitor the in situ production of H2O2 induced by Aβ aggregates in neuronal cells. Most notably, NPBZ has the ability to penetrate the blood-brain barrier and to simultaneously image Aβ plaques and H2O2 in the brain of AD mice of different ages via the two independent channels in vivo. The dual-functional detection performance makes NPBZ useful in the diagnostic study of AD, which may provide direct elucidations for the roles of Aβ plaques and H2O2 in the pathogenesis of AD. Download figure Download PowerPoint Previous articleNext article FiguresReferencesRelatedDetails Issue AssignmentNot Yet AssignedSupporting Information Copyright & Permissions© 2025 Chinese Chemical Society Downloaded 0 times PDF downloadLoading ...