曲古抑菌素A
染色质免疫沉淀
下调和上调
基因敲除
癌症研究
HDAC1型
化学
分子生物学
细胞生长
组蛋白脱乙酰基酶
免疫沉淀
组蛋白
生物
基因表达
发起人
基因
生物化学
作者
Nanzi Xie,Sisi Mei,Changlan Dai,Wei Chen
标识
DOI:10.1615/critreveukaryotgeneexpr.2023051396
摘要
HDAC1 functions as an oncogene in multi-type cancers. This study aimed to investigate the roles of histone deacetylase 1 (HDAC1) in cervical cancer (CC). mRNA expression was determined using reverse transcription quantitative polymerase chain reaction. The protein-protein complexes was analyzed using co-immunoprecipitation assay. The binding sites between NRF2 and NEU1 were confirmed by chromatin immunoprecipitation assay. Cell viability was detected by CCK-8. Cell proliferation was measured using CCK-8 and colony formation assays. Cell migrative and invasive ability were determined using transwell assay. We found that HDAC1 was upregulated in CC patients and cells. Trichostatin A (TSA) treatment decreased the number of colonies and migrated and invaded cells. Moreover, HDAC1 interacted with NRF2 to downregulate NEU1 expression. NEU1 knockdown attenuated the effects of TSA and enhanced the aggressiveness of CC cells. In conclusion, HDAC1 functions as an oncogene in CC. Targeting HDAC1 may be an alternative strategy for CC.
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