Comparative characterisation of a commercial human chorionic gonadotrophin extracted from human urine with a commercial recombinant human chorionic gonadotrophin

重组DNA 尿 人绒毛膜促性腺激素 医学 男科 内分泌学 内科学 生物 生物化学 激素 基因
作者
RM Bassett,Claire Bellis,L. Chiacchiarini,Diego La Mendola,E. Micangeli,Katia Minari,Luigi Maria Grimaldi,Michele Mancinelli,Renato Mastrangeli,Roberto Bucci
出处
期刊:Current Medical Research and Opinion [Taylor & Francis]
卷期号:21 (12): 1969-1976 被引量:21
标识
DOI:10.1185/030079905x75005
摘要

ABSTRACTBackground: A commercial preparation of recombinant human chorionic gonadotrophin (r-hCG, Ovitrelle) was launched in 2001. Generally, hCG is available in two formats: human chorionic gonadotrophin (u-hCG), derived from the urine of pregnant females, and r-hCG produced by DNA based biotechnology. * Ovitrelle is a trade name of Serono International SA, Geneva, SwitzerlandMethod: The analytical characteristics of a highly purified u-hCG (Gonasi HP) were assessed and compared, for the first time, with the recombinant derived r-hCG (Ovitrelle). Gonasi HP is produced by extracting and purifying hCG from urine to obtain a specific bioactivity of 5000 IU/mg protein. Ovitrelle is produced via a recombinant derived mammalian cell line and purified to obtain a specific activity of 26 000 IU/mg. † Gonasi HP is a trade name of IBSA, Lugano, SwitzerlandResults and conclusion: It has been documented that commercially available u-hCG preparations can contain a number of urine derived protein contaminants as well as hCG related metabolites. This is also the case for Gonasi HP, where hCG related molecules and other proteins were found to be present, including epidermal growth factor (EGF) and eosinophil derived neurotoxin (EDN). It was also demonstrated that this preparation contained high levels of oxidised hCG. r-hCG was confirmed to be essentially intact hCG, free from contaminant proteins and with very low levels of oxidised hCG. Key words: : Chorionic gonadotrophin (hCG)Highly purified urine derived hCG (u-hCG)Recombinant human chorionic gonadotrophin (r-hCG) Notes* Ovitrelle is a trade name of Serono International SA, Geneva, Switzerland† Gonasi HP is a trade name of IBSA, Lugano, Switzerland
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