RNA干扰
程序性细胞死亡
腺苷酸激酶
生物
细胞生物学
HEK 293细胞
背景(考古学)
细胞生长
激酶
细胞培养
核糖核酸
细胞凋亡
基因
遗传学
生物化学
酶
古生物学
作者
Fanzhi Kong,Bert Binas,Jae-Hee Moon,Sung Soo Kang,Hyo Joon Kim
标识
DOI:10.1016/j.abb.2013.02.014
摘要
Adenylate kinase isozyme 4 (AK4) belongs to a family of nucleotide monophosphate kinases involved in energy metabolism. Recently, AK4 was reported to play a role in protection from stress: In HEK293 cells, hypoxia increases AK4 expression but does not affect proliferation or viability, while RNA interference (RNAi) directed against AK4 inhibits proliferation and promotes death. By contrast, we show here that HepG2 cells showed much higher AK4 levels, which decreased under hypoxia along with markedly reduced cell proliferation and increased cell death. Nevertheless, RNAi directed against AK4 inhibited cell proliferation and caused death in both cell types, although cell cycle parameters were affected only in HepG2 cells. Hence reductions of AK4 levels were always associated with cell death. These results extend the notion of a stress-protective function of AK4 to a novel physiological context and show that AK4-mediated stress protection is not limited to one particular death scenario. Our data also allow the hypothesis that the different basal AK4 levels reflect different basal stress levels, causing alternative responses to additional stress.
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