TLR5型
鞭毛蛋白
细胞生长
癌细胞
癌症研究
生物
MAPK/ERK通路
癌症
Toll样受体
细胞生物学
受体
信号转导
免疫学
免疫系统
先天免疫系统
生物化学
遗传学
作者
Eun‐Jung Song,Min‐Jung Kang,Yong-Seok Kim,Sun-Moon Kim,Sang-Eok Lee,Chang-Hwan Kim,Dong‐Jae Kim,Jong‐Hwan Park
标识
DOI:10.3892/ijmm.2011.656
摘要
Signaling of the Toll-like receptor (TLR) is closely associated with tumor development and progression processes including cell proliferation, angiogenesis, metastasis, and immunosuppression. In this study, we examined the expression of TLR5 in gastric cancer cells and its function in cell proliferation. RT-PCR revealed that the TLR5 gene was expressed in all gastric cancer cell lines examined, SNU638, SNU601, SNU216, and AGS. The TLR5 agonist, flagellin, induced IL-8 production and NF-κB activation in the gastric cancer cell lines. In addition, flagellin enhanced the proliferation of all gastric cancer cells examined, whereas LPS did not affect that of SNU638 cells. Blockade of TLR5 using an antibody, restored the proliferation of SNU638 cells enhanced by flagellin, indicating that TLR5 is essential for cell proliferation by flagellin. Flagellin also led to phosphorylation of ERK in SNU638 cells. The ERK inhibitor, PD98059, restored the proliferation ability of SNU638 cells enhanced by flagellin, suggesting that ERK may play an important role in the proliferation of gastric cancer cells. These findings suggest that TLR5 may play an important role in tumor progression of gastric cancer via the regulation of cell proliferation.
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