Differentiation of Porcine Reproductive and Respiratory Syndrome Virus N Protein Using a Virus-based ELISA

The bacterially expressed nucleocapsid (N) protein of porcine respiratory and reproductive syndrome virus (PRRSV) was used as immunogen to generate a rabbit-derived polyclonal antibody. The immunoreactivity of the protein to the antibody was confirmed by Western blot analysis. Using PRRSV, transmissible gastroenteritis virus, porcine epidemic diarrhea virus, pseudorabies virus, and avian infectious bronchitis virus as coating antigens, a virus-based ELISA was established. The polyclonal antibody against PRRSV N protein used as a diagnostic agent was capable of differentiating PRRSV from the other viruses.