纳米孔测序
纳米孔
DNA
DNA测序
支原体
核苷酸
结扎测序
生物
计算生物学
生物物理学
DNA测序器
仆从
基因组
单细胞测序
大规模并行测序
霰弹枪测序
化学
桑格测序
遗传学
纳米技术
基序列
基因
基因组文库
材料科学
医学
病理
结核分枝杆菌
肺结核
作者
Ian M. Derrington,Tom Z. Butler,Marcus D. Collins,Elizabeth A. Manrao,Mikhail Pavlenok,Michael Niederweis,Jens H. Gundlach
标识
DOI:10.1073/pnas.1001831107
摘要
Nanopore sequencing has the potential to become a direct, fast, and inexpensive DNA sequencing technology. The simplest form of nanopore DNA sequencing utilizes the hypothesis that individual nucleotides of single-stranded DNA passing through a nanopore will uniquely modulate an ionic current flowing through the pore, allowing the record of the current to yield the DNA sequence. We demonstrate that the ionic current through the engineered Mycobacterium smegmatis porin A, MspA, has the ability to distinguish all four DNA nucleotides and resolve single-nucleotides in single-stranded DNA when double-stranded DNA temporarily holds the nucleotides in the pore constriction. Passing DNA with a series of double-stranded sections through MspA provides proof of principle of a simple DNA sequencing method using a nanopore. These findings highlight the importance of MspA in the future of nanopore sequencing.
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