聚酮合酶
化学
聚酮
基质(水族馆)
酶
生物化学
立体化学
体外
ATP合酶
发酵
底物特异性
生物合成
组合化学
生物
生态学
作者
James S. Parascandolo,Judith Havemann,Helen K. Potter,Fanglu Huang,Elena Riva,Jack A. Connolly,Ina Wilkening,Lijiang Song,Peter F. Leadlay,Manuela Tosin
标识
DOI:10.1002/anie.201509038
摘要
Abstract Chemical probes capable of reacting with KS (ketosynthase)‐bound biosynthetic intermediates were utilized for the investigation of the model type I iterative polyketide synthase 6‐methylsalicylic acid synthase (6‐MSAS) in vivo and in vitro. From the fermentation of fungal and bacterial 6‐MSAS hosts in the presence of chain termination probes, a full range of biosynthetic intermediates was isolated and characterized for the first time. Meanwhile, in vitro studies of recombinant 6‐MSA synthases with both nonhydrolyzable and hydrolyzable substrate mimics have provided additional insights into substrate recognition, providing the basis for further exploration of the enzyme catalytic activities.
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