预定位
脂质体
钆
血管生成
化学
单克隆抗体
癌症研究
磁共振成像
内皮糖蛋白
医学
分子生物学
核磁共振
抗体
免疫学
生物
生物化学
细胞生物学
放射科
有机化学
物理
放射免疫疗法
干细胞
川地34
作者
Lina Qiu,Jiawen Zhang,Shuping Li,Cao Xie,Zhenwei Yao,Xiaoyuan Feng
摘要
Purpose To evaluate the use of endoglin‐targeted paramagnetic liposomes in delineating the glioma margins using magnetic resonance (MR) angiogenesis imaging in a rat model. Materials and Methods Four liposome preparations, including nontargeted paramagnetic liposomes (Gd‐SLs), isotype control IgG‐coupled paramagnetic liposomes (IgG‐Gd‐SLs), endoglin monoclonal antibody coupled paramagnetic liposomes (MAb‐Gd‐SLs), and biotinylated antibodies (Bio‐MAb)/streptavidin‐coupled paramagnetic liposomes (SAv‐Gd‐SLs) for two‐step pretargeting imaging, were formulated. All animal experiments were carried out with the approval of the Shanghai Animal Care. C6 glioma‐bearing Sprague‐Dawley rats were intravenously injected with gadolinium‐diethylenetriaminepentaacetic acid (Gd‐DTPA) or the previously mentioned liposomes ( n = 5) and imaged with MR. T 1 ‐weighted MRI was performed before and dynamically repeated after different contrast agents were injected. The enhancement features of the tumors were compared. Results The signal enhancement of the tumor in the two‐step pretargeting group increased by 117.9 ± 5.3% at the periphery and 109.2 ± 3.5% in the center ( P = 0.032) at the 8‐hour timepoint after SAv‐Gd‐SLs injection. Ring‐like enhancement margins were demonstrated at the periphery of the tumor in the two‐step targeted group. The specificity of the targeted liposomes was supported by the competitive study. The signal of peak enhancement using MAb‐Gd‐SLs was 59% less than that of the two‐step group and only slightly higher than the non‐targeted groups. Conclusion The two‐step endoglin‐targeted imaging using biotin‐streptavidin interaction was demonstrated to induce intense enhancement of the tumor periphery, which implies that this advanced MR molecular contrast agent may be suitable for accurately delineating glioma tumor margins. J. Magn. Reson. Imaging 2015;41:1056–1064 . © 2014 Wiley Periodicals, Inc .
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