透明质酸
脚手架
再生(生物学)
细胞外基质
脊髓
细胞生物学
组织工程
脐静脉
细胞生长
血管生成
成纤维细胞生长因子
化学
解剖
生物医学工程
生物化学
体外
生物
神经科学
医学
癌症研究
受体
作者
Ao Huang,Ying Huang,Wen Yang,Lu Wang,Renchuan You,Jiannan Wang,Shuqin Yan,Qiang Zhang
标识
DOI:10.1016/j.ijbiomac.2024.130287
摘要
Bioactive scaffolds accurately mimicking the structure and composition of the extracellular matrix have garnered significant interest in tissue engineering. In this study, we developed a platform utilizing natural silk nanofibrils, hyaluronic acid, and basic fibroblast growth factor for the purpose of promoting spinal cord regeneration by creating an optimal microenvironment. The bioactive scaffold exhibited notable characteristics such as high porosity and hydrophilicity, attributed to its unique nanostructure, high connectivity, and polysaccharide composition. Furthermore, the pore size of the scaffold can be adjusted within the range of 90 μm to 120 μm by varying the content of hyaluronic acid. In vitro, human umbilical vein endothelial cells were seeded into the scaffold, demonstrating enhanced cell viability. The scaffold facilitated cell proliferation and migration. In vivo experiments on rats indicated that the scaffold had a beneficial impact on spinal cord regeneration, creating a conducive environment for motor function recovery of the rats. This effect may be attributed to the scaffold's ability to stimulate axon growth and neuronal survival, as well as inhibit the formation of glial scars, as evidenced by the decreased expression of growth associated protein-43, microtubule-associated protein 2, and neurofilament-200. This study presents a promising method to develop a feasible bioscaffold for the treatment of spinal cord injury.
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