Harnessing the catalytic plasticity of the ent-kaurene synthase from Bradyrhizobium japonicum to produce the ent-rosane and ent-pimarane scaffolds

The F72Y mutation of the ent -kaurene synthase from Bradyrhizobium japonicum leads to the formation of ent -rosa-5(10),15-diene and ent -pimara-8,15-diene, two common scaffolds of labdane diterpenoids.