Effects of abamectin on nonspecific immunity, antioxidation, and apoptosis in red swamp crayfish (Procambarus clarkii)

克氏原螯虾 阿维菌素 生物 超氧化物歧化酶 小龙虾 过氧化氢酶 抗氧化剂 毒理 生物化学 杀虫剂 生态学
作者
Tianyu Guan,Jianbin Feng,Quanxin Zhu,Long Wang,Ping Xie,Hui Wang,Jiale Li
出处
期刊:Fish & Shellfish Immunology [Elsevier BV]
卷期号:142: 109137-109137
标识
DOI:10.1016/j.fsi.2023.109137
摘要

Abamectin, a pesticide of 16-member macrocyclic lactones, is widely applied in agriculture. As an important environmental factor, pesticides pose a great threat to defense system in aquatic animals. Procambarus clarkii is one of the most important economic aquatic animals in China. It is necessary to explore the defense mechanism of P. clarkii to abamectin. In this study, P. clarkii were exposed to 0, 0.2, 0.4, 0.6 mg/L abamectin, immune- and antioxidant-related enzymes activities, genes expression levels, and histological observations were used to analyze the defense capacity of P. clarkii to abamectin. With increasing abamectin concentration, reactive oxygen species (ROS) level and malondiadehyde (MDA) content increased significantly. Meanwhiile, acid phosphate (ACP), alkaline phosphatase (AKP) activities, total haemocyte counts (THC), and Crustin expression level decreased significantly, superoxide dismutase (SOD), catalase (CAT) activities, total antioxidant capacity (T-AOC), and GPX expression level also decreased significantly. Hematoxylin & eosin (H&E) observation showed that with increasing abamectin concentration, hepatopancreas were damaged, especially membrane structure. Through TUNEL observation and apoptosis-related genes (PcCTSL, Bcl-2, Bax, BI-1, PcCytc, caspase-3) expression levels, with increasing abamectin concentration, apoptosis rate increased significantly. Results of this study indicated that abamectin caused oxidative damage to P. clarkii, resulting in damage to defense system, suppression of nonspecific immunity and antioxidation, and promotion of apoptosis. It provided theoretical basis for healthy P. clarkii culture, and for further study on defense mechanism of aquatic animals to pesticides.
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